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Human Prolactin (PRL: lactogenic hormone) is secreted from the anterior pituitary gland in both men and women. PRL is an estrogen-related rise at puberty and a corresponding decrease atmenopause. During pregnancy, PRL level increases progressively to 10 and 20 times of normal value, declining to non-pregnant levels by 3-4 weeks post-partum. The determination of PRL concentration is helpful in diagnosing hypothalamic-pituitary disorders. Microadenomas (small pituitary tumors) may causehyperprolactinemia, which is sometimes associated with male impotence.
ichroma™ PRL Test is based on fluorescence immunoassay technology. ichroma™ PRL uses a sandwich immunodetection method, such that by mixing detector buffer with blood specimen in test vial, the fluorescence-labeled detector anti-PRL antibody in buffer binds to PRL antigen in blood specimen. The sample mixture is loaded and migrates on the matrix of test cartridge; the complexes of detector antibody and PRL are captured to anti-PRL sandwich pair antibody that has been immobilized on test matrix.