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Myoglobin is an iron- and oxygen-binding protein found in bothskeletal and myocardial muscles. It acts as a transport protein and is involved in diffusion of oxygen in the muscle tissue. Myoglobin isreleased into the serum more rapidly as compared to other cardiac markers upon damage to the myocardial cells. Serum concentration of myoglobin increases above the normal range as early as 1 hour after acute myocardial infarction (AMI), attainspeak level in approximately 4 to 8 hours after the onset and normalize rapidly afterwards. Thus myoglobin is better suited as acardiac marker for early diagnosis of AMI.
ichroma™ Myoglobin Test is based on fluorescence immunoassay technology. ichroma™ Myoglobin uses a sandwich immunodetection method, such that by mixing detector buffer with blood specimen in test vial, the fluorescence-labeled detector anti-Myoglobin antibody in buffer binds to Myoglobin antigen in blood specimen. The sample mixture is loaded and migrates on the matrix of test cartridge; the complexes of detector antibody and Myoglobin are captured to anti-Myoglobin sandwich pair antibody that has been immobilized on test matrix.