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Human chorionic gonadotropin (hCG) is a glycoprotein hormone secreted by the developing placenta shortly after implantation. hCG can be detected in the urine and serum of pregnant women as early as 6 to 15 days after conception. The concentration of hCG increases to 50 mIU/ml one week post implantation and reaches to about 100 mIU/ml at the time of the first missed menstrual periodand the peak at 100,000-200,000 mIU/ml at the first trimester.
ichroma™ hCG Test is based on fluorescence immunoassay technology. ichroma™ hCG uses a sandwich immunodetection method, such that by mixing detector buffer with blood specimen in test vial, the fluorescence-labeled detector anti-hCG antibody in buffer binds to hCG antigen in blood specimen. The sample mixture is loaded and migrates on the matrix of test cartridge; the complexes of detector antibody and hCG are captured to anti-hCG sandwich pair antibody that has been immobilized on test matrix.