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Alpha-fetoprotein (AFP) is a 1-globulin family of human plasma proteins. AFP is produced primarily in the liver of developing fetus. A great increase of AFP concentration in several malignant diseases mostly is primary hepatocellular carcinoma and non-seminomatous testicular cancer. Some 70-90% of patients with primary hepatocellular carcinoma and non-seminomatous testicular cancer have been observed to have high levels of AFP.
ichroma™ AFP Test is based on fluorescence immunoassay technology. ichroma™ AFP uses a sandwich immunodetection method, such that by mixing detector buffer with blood specimen in test vial, the fluorescence-labeled detector anti-AFP antibody in buffer binds to AFP antigen in blood specimen. The sample mixture is loaded and migrates on the matrix of test cartridge; the complexes of detector antibody and AFP are captured to anti-AFP sandwich pair antibody that has been immobilized on test matrix.