SALSA MLPA kits for copy number quantification

MLPA ^® is the gold standard for DNA copy number quantification and is used to study both hereditary disorders and tumours. MLPA can also be used to determine if a known mutation is present in the DNA or to investigate the methylation status of DNA sequences. MRC-Holland, the inventor and exclusive manufacturer of MLPA assays, offers more than 400 different MLPA panels used for DNA copy number quantification, mutation identification, and DNA methylation status. 

Popular MLPA applications

• Predisposition to Cancer

  • BRCA1, BRCA2, MLH1, MSH2, MSH6, PMS2, APC, MMR, NF1

• Neuromuscular Disorders

  • SMA, DMD, CMT1

• Intellectual Disability

  • Microdeletion, PWS/AS, FMR1/AFF2, MECP2, Sotos, WBS, SHOX, aneuploidy

• Solid Tumours

  • Gliomas, MMR, Neuroblastoma, TP53, Breast

• Methylation profiling

  • Prader-Willi / Angelman syndrome, Fragile X, Beckwith-Wiedemann Syndrome

Advantages and features of MLPA

• Examine up to 60 targets per reaction

• Only 50ng of DNA is required per reaction

• MLPA detects a short sequence of 60-80 nucleotides

• Detection of single exon deletions and duplications

• Detection of targeted point mutations

• One simple protocol for >400 applications

• Results available within 24 hours

• Limited lab equipment required: only a thermocycler and a sequence type electrophoresis system

• User-friendly, reliable, and free data analysis software, Coffalser.Net ^TM

Visit the MLPA website www.mlpa.com to:

• Search for genes, and chromosomal regions of interest to find all related MLPA products

• Explore the MLPA procedure to learn how to do MLPA

• Get MLPA support by viewing education, workshop, and troubleshooting material

• Stay up to date with current MLPA news

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